1)
Which
of the following enzyme is not involved in galactose metabolism?
a)
Glucokinase
b)
Galactokinase
c)
Galactose-1-Phosphate
Uridyl transferase
d)
UDP-Galactose
4- epimerase
2)
Which
of the following enzyme is defective in galactosemia- a fatal genetic disorder
in infants?
a) Glucokinase
b) Galactokinase
c) Galactose-1-Phosphate Uridyl
transferase
d) UDP-Galactose 4- epimerase
3)
In
the liver, the accumulation of which of the following metabolite attenuates the
inhibitory of ATP on phosphofructokinase?
a)
Glucose-6-Phosphate
b)
Citrate
c)
Fructose-1,6-Bisphosphate
d)
Fructose-2,6-Bisphosphate
4)
Mutation
in which of the following enzymes leads to a glycogen storage disease known as
Tarui’s disease?
a) Glucokinase
b)Phosphofructokinase
c) Phosphoglucomutase
d) Pyruvate Kinase
5)
Erythrocytes
undergo glycolysis for the production of ATP. The deficiency of ……………. enzyme
leads to hemolytic anemia?
a) Glucokinase
b)Phosphofructokinase
c) Phosphoglucomutase
d) Pyruvate Kinase
6)
Cancer
cells have high energy demands for replication and division. Increased flux of
glucose into glycolysis replenishes the energy demand. Which of the following enzyme plays an
important role in tumor metabolism?
a) Glucokinase
b)Phosphofructokinase
c) Phosphoglucomutase
d) Pyruvate Kinase M2
7)
Which
of the following glucose transporter (GLUT) are important in insulin-dependent
glucose uptake?
a) GLUT1
b) GLUT2
c) GLUT3
d) GLUT4
8)
Which
of the following glucose transporter (GLUT) is present in beta cells of the
pancreas?
a) GLUT1
b) GLUT2
c) GLUT3
d) GLUT4
9)
Which
of the following glucose transporter (GLUT) is important in fructose transport
in the intestine?
a) GLUT1
b) GLUT3
c) GLUT5
d) GLUT7
10)
Which
of the following metabolite negatively regulates pyruvate kinase?
a) Fructose-1,6-Bisphosphate
b) Citrate
c) Acetyl CoA
d) Alanine
11)
In
absence of oxygen, pyruvate is converted into lactate in muscle because
a) Lactate is the substrate from the
downstream pathway
b) Lactate acts as a substrate for
the formation of amino acid
c) during the product of lactate two
ATP are produced
d) during lactate formation, NADH is
reconverted into NAD.
12)
Which
of the following glycolytic enzyme is inhibited by the accumulation of
long-chain fatty acid in the liver?
a) Hexokinase
b) Glucokinase
c)Phosphofructokinase
d) Pyruvate kinase
13)
Which
of the following statement related to phosphofructokinase-I is false:
a) PFK-2 is the isoenzyme of PFK-1
that is present in the liver
b) PFK-1 is activated by AMP whereas
inhibited by ATP and citrate
c) The binding of ATP to PFK-1
induces the conformation change from R to T state
d) PFK-1 is regulated by
posttranslational modification such as phosphorylation
14)
Which
of the following statement about Phosphofructokinase-2 (PFK-2) is false?
a) PFK-2 is a bifunctional enzyme
having a kinase domain, phosphatase domain, and a regulatory domain
b) Activated protein kinase A
phosphorylates PFK-2 and activates phosphatase domain
c) PFK-2 catalyzes the conversion of
fructose-6-phosphate to fructose 2-6 bisphosphate
d) PFK-2 phosphatase activity is
activated by the insulin signaling pathway.
15)
Which
of the following hormone decreases blood glucose and increases the uptake of
glucose in various tissues like skeletal muscle, adipose tissues?
a) Glucagon
b) Epinephrine
c) Cortisol
d) Insulin
16)
Which
of the following statement is true?
a) Glycolysis occurs only in
mammalian cells
b) Glycolysis occurs in mitochondria
c) Glycolysis occurs in the presence
and absence of oxygen
d) Glycolysis occurs when ATP
concentration is high.
17)
What
is the rate-limiting step in glycolysis?
a) Hexokinase
b) Phosphohexose isomerase
c) Glyceraldehyde-3-phosphate
dehydrogenase
d) Enolase
18)
The
net gain of ATP during the conversion of glucose to pyruvate is:
a) 1 ATP
b) 2 ATP
c) 1 ATP +1 GTP
d) 4 ATP
19)
During
the conversion of glucose to pyruvate, two NADH molecules are generated.
Which of the following steps
generates NADH?
a) Conversion of fructose-6-phosphate
to fructose-1-6-bisphosphate
b) Conversion of
glyceraldehydes-3-phosphate to 1-3-bisphosphoglycerate
c)Conversion of 3-phosphoglycerate
to 2-phosphoglycerate
d) Conversion of phosphoenolpyruvate
to pyruvate
20)
What
is the committed step in glycolysis?
a) Conversion of glucose to
glucose-6-phosphate
b) Conversion of
fructose-6-phosphate to fructose-1,6-bisphosphate
c) Conversion of
glyceraldehydes-3-phosphate to 1-3-bisphosphoglycerate
d) Conversion of 3-phosphoglycerate
to 2-phosphoglycerate
21)
Glycolysis
consists of three irreversible steps.
Which of the following
enzyme-catalyzed reaction are not irreversible steps in glycolysis?
a) Hexokinase
b) Phosphofructokinase
c) Glyceraldehyde-3-phosphate kinase
d) Pyruvate kinase
22)
The
following are the negative regulators of phosphofructokinase except
a) ATP
b) AMP
c) Citrate
d) pH
23)
Which
of the following step is inhibited by sodium fluoride?
a) Glyceraldehyde-3-phosphate
dehydrogenase
b) 3-phosphoglycerate mutase
c) Enolase
d) AMP
24)
Which
of the following step is inhibited during arsenate poisoning?
a) Glyceraldehyde-3-phosphate
dehydrogenase
b) 3-phosphoglycerate mutase
c) Enolase
d) Pyruvate kinase
25)
Which
of the following enzyme catalyzes the conversion of pyruvate to lactate?
a) Pyruvate reductase
b) Lactate reductase
c) Lactate dehydrogenase
d) Pyruvate dehydrogenase
26)
Glucokinase
is an isoenzyme of hexokinase that has high Km and Vmax.
Which of the following organ
expresses glucokinase?
a) Kidney
b) Muscle
c) Liver
d) Brain
27)
Gluconeogenesis
is the production of glucose from non-carbohydrate molecules.
Which of the following is not
substrate for gluconeogenesis?
a) Lactate
b) Alanine
c) Glycerol
d) Acetyl CoA
28)
Gluconeogenesis
occurs in the liver and kidneys.
Which is of the following enzyme are
important for gluconeogenesis are expressed exclusively in these tissues?
a) Glucose-6-phosphatase
b) Fructose-1,6-Bisphosphatase
c) Phosphoenolpyruvate carboxykinase
d) Pyruvate carboxylase
29)
During
gluconeogenesis, the three irreversible steps of glycolysis have to be
bypassed. The first step is the conversion of pyruvate to phosphoenolpyruvate.
Which of the following statement is
false regarding the reaction step?
a) This reaction involves a two-step
process catalyzed by pyruvate carboxylase and phosphoenolpyruvate carboxykinase
b) Conversion of oxaloacetate from
pyruvate occurs in mitochondria and shuttled into the cytosol.
c) Formation of phosphoenolpyruvate
requires both ATP and GTP as an energy source.
d) Acetyl CoA is an activator of the
enzyme pyruvate carboxylase.
30)
During
gluconeogenesis, the three irreversible steps of glycolysis have to be
bypassed. The final step is the conversion of glucose-6-P to glucose that is
catalyzed by glucose-6-phosphatase.
Which of the following statement is
true about the reaction step?
a) Conversion of glucose-6-phosphate
to glucose releases one ATP molecule
b) It is a highly active enzyme in
skeletal muscle
c) Defect in glucose-6-phosphatase
leads to abnormal accumulation of glycogen in the liver
d) The reaction occurs in
mitochondria
31)
Which
of the following statement is true about Cori Cycle?
a) The Cori cycle involves three
tissues muscle, liver, and brain
b) It involves the transport of
lactate from the liver to skeletal tissue for gluconeogenesis
c) It involves the transport of
lactate from skeletal muscle to the liver for gluconeogenesis
d) It is active during resting
stages and well-fed condition
32)
During
prolong starvation, which of the following hormone is responsible for
increasing gluconeogenesis in the liver
a) Insulin
b) Glucagon
c) TSH
d) Thyroxine
Answers:
1-a)
Glucokinase
2-c)
Galactose-1-Phosphate Uridyl transferase
3-d)
Fructose-2,6-Bisphosphate
4-b)Phosphofructokinase.
5-
d) Pyruvate Kinase
6-
d) Pyruvate Kinase M2
7-
d) GLUT4
8- b)
GLUT2
9-
c) GLUT5
10-
d) Alanine
11-
d) during lactate formation, NADH is reconverted into NAD
12-
b) Glucokinase
13-
d) PFK-1 is regulated by posttranslational modification such as phosphorylation
14-
b) Activated protein kinase A phosphorylates PFK-2 and activates phosphatase
domain
15-d)
Insulin
16-c)
Glycolysis occurs in presence and absence of oxygen
17-
a) Hexokinase
18-
b) 2 ATP
19-
b) Conversion of glyceraldehydes-3-phosphate to 1-3-bisphosphoglycerate
20-
b) Conversion of fructose-6-phosphate to fructose-1,6-bisphosphate
21-
c) Glyceraldehyde-3-phosphate kinase
22-b)
AMP
23-
c) Enolase
24-a)
Glyceraldehyde-3-phosphate dehydrogenase
25-
c) Lactate dehydrogenase
26-c)
Liver
27-d)
Acetly CoA
28-
a) Glucose-6-phosphatase
29-d)
Acetyl CoA is an activator of enzyme pyruvate carboxylase
30-
c) Defect in glucose-6-phosphatase leads to abnormal accumulation of glycogen
in liver
31-c)
It involves transport of lactate from skeletal muscle to liver for
gluconeogenesis
32-b)
Glucagon